The Brazo del Este is located 20 km south of Seville in the Guadalquivir river estuary, which is an area with one of the highest levels of biodiversity in the Iberian peninsula. This former branch (brazo) of the Guadalquivir is east (este) of the river and is now a reed-fringed lagoon and wetland area surrounded by rice plains and farmland.

Situated to the south of Seville, on the N-IV old Cadiz road, Dos Hermanas is the second-biggest and most important town by population in Seville province, and the ninth-largest town in Andalucia, with about 120,000 inhabitants.